A critical comparison of the T-1824 dye and iodinated albumin methods for plasma volume measurement.
نویسندگان
چکیده
The T-1824 dye method for plasma volume measurement has been subjected to much criticism. The validity of its use as a tagged protein dilution method has been questioned. One of the major criticisms has been that part of the injected dye is rapidly removed from the plasma before binding with protein occurs. This objection does not apply to the use of human serum albumin tagged with radioactive iodine before injection. Comparison of the two methods has given conflicting results (1-3). The present study was undertaken to clarify the relationship between the dye and the iodinated albumin methods for the measurement of the plasma volume.
منابع مشابه
Simultaneous measurement in dogs of plasma volume with I131 human albumin and T-1824 with comparisons of their long-term disappearance from the plasma.
vv HEN used for measurement of plasma volume, bovine plasma proteins (I) and dog hemoglobin (2) have the same initial volume distribution as the dye, T-1824, which in the bloodstream combines preferentially with albumin (3). In regard to simultaneous comparisons in dogs of plasma volume measured with T-1824 and human albumin labeled with the 1131 isotope, there are conflicting reports (cf. 4-7)...
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Investigation of the properties of indigo carmine reveals that it may be more suitable than the dye, T-1824, for circulator}' studies that are now commonly carried out using the dye dilution principle of Stewart and Hamilton. Comparison is made of simultaneous dilution curves of indigo carmine and radioactive iodinated serum albumin. Apparently, indigo carmine is bound to protein firmly enough ...
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The dye T-1824 (Evans Blue) has gained wide acceptance for determining plasma volume. Recently there has been a renewed interest in dye circulation studies for the measurement of cardiac output, central volume, cardiac shunts, and valvular regurgitation. The method of estimation most commonly used is spectrophotometric analysis of the dye-tinged plasma (or serum) compared with standard dye solu...
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Studies of the mechanism of proteinuria and particularly of albuminuria require the application of accurate methods to the determination of the concentrations of individual proteins in plasma and urine. Electrophoresis is not practical because of the very large number of analyses necessary. The availability of human plasma albumin (i.e., fraction V) has permitted extensive use of immunological ...
متن کاملSimultaneous distribution of T-1824 and I131 labelled human serum albumin in man.
The dilution of injected dyes (1-3), especially Evans Blue (T-1824) (4), has gained wide acceptance as a measure of plasma volume (5, 6). Recently, such an interpretation of the "T-1824 space" has been questioned. The challenges are based largely upon discordant volumes of distribution obtained after the simultaneous administration of T-1824 and labelled red blood cells (7-14), or T-1824 and ra...
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عنوان ژورنال:
- The Journal of clinical investigation
دوره 32 2 شماره
صفحات -
تاریخ انتشار 1953